SKIN GRAFTS
INTRODUCTION
Plastic surgery, although thought of as a technique-oriented specialty, is in fact a problem-solving field. The training of a plastic surgeon allows him or her to see surgical problems in a different light and select from a variety of options to solve surgical problems. Plastic surgeons have received broad training, and most have completed residencies in other fields such as general surgery, ENT surgery, orthopedics, urology, or neurosurgery.
The basic principles of plastic surgery are careful analysis of the surgical problem, careful planning of procedures, precise technique, and atraumatic handling of tissues. Alteration, coverage, and transfer of skin and associated tissues are the most common procedures performed. Plastic surgery may deal with closure of surgical wounds—particularly recalcitrant wounds such as those occurring post radiation or poorly healing wounds in immunocompromised patients—removal of skin tumors, repair of soft tissue injuries or burns, correction of acquired or congenital deformities of the breast, or repair of cosmetic defects. Operations on the head and neck and the hand may require special surgical training.
In the past 2 decades, increased knowledge of anatomy and the development of many new techniques have brought about important changes in plastic surgery. It is now known that in many areas the blood supply of the skin is derived principally from vessels arising from underlying muscles and larger perforating blood vessels rather than solely from vessels of the subcutaneous tissue, as was formerly thought. One-stage transfer of large areas of skin and muscle tissue can be accomplished if the axial pedicle of the underlying muscle is included in the transfer. With the use of microsurgical techniques, musculocutaneous units or combinations of bone, muscle, and skin can be successfully transferred and vessels and nerves less than 1 mm in size can be repaired. These so-called free-flap transplantations are a major advance in the treatment of defects that were previously untreatable or required lengthy or multistaged procedures. More sophisticated knowledge of the blood supply to the skin has introduced a concept of perforator flaps whereby one perforating vessel is identified that may supply a large segment of overlying skin. Similarly, the concept of neurocutaneous arteries and flaps has given rise to the design of additional flap territories such as the so-called sural flap in the lower leg.
The plastic surgeon, as a member of the craniofacial surgical team, is able to dramatically improve the appearance and function of children with severe congenital deformities. Children of normal intelligence who previously had been social outcasts are now able to lead relatively normal lives. Improved understanding of facial growth and abnormal development and diagnostic techniques such as the CT scan, MRI, and three-dimensional computer-assisted imaging enable the reconstructive surgeon to develop a complex strategy for remodeling the deformed craniofacial skeleton. This may involve remodeling or repositioning part or all of the cranial vault, the orbits, the mid face, and the mandible. These complex and at times formidable reconstructions, which were performed by moving both units and adding bone grafts from the ileum, have more recently been somewhat simplified with the introduction of the miniplate method of fixation as well as of bone substitutes that may serve as a scaffold upon which normal bone can regrow.
A recent notable advance in craniofacial surgery has been the introduction of distraction osteogenesis, which borrows from the Ilizarov principle of distraction where one makes a cortical cut in the bone and then applies a distraction apparatus so that in measured amounts (usually 1 mm per day) the bone is transported to bridge a gap. In craniofacial surgery it is more commonly brought to bear to enlarge or cause overgrowth of areas such as an underdeveloped mandible.
Additional areas of involvement for the plastic surgeon entail allotransplantation, particularly with the increasing number of clinical limb allotransplants, which unfortunately at present still require immunosuppression. It is hoped that immunotolerance will some day become a reality, thus allowing transplantation of nonessential organs.
Tissue engineering of bone, cartilage, and nerve is an area of ongoing research for plastic surgeons. Although encouraging experimental results have been reported in anatomic areas difficult to reconstruct such as the external ear, there are as yet no clinical applications.
Fetal surgery—an area pioneered by a number of plastic surgeons—appears to be in a quiescent stage, particularly because of advances in the treatment of cleft lip and cleft palate in the newborn.
Jones JW et al: Successful hand transplantation. One-year follow-up. Louisville Hand Transplant Team. N Engl J Med 2000;343:468.
Plastic surgery, although thought of as a technique-oriented specialty, is in fact a problem-solving field. The training of a plastic surgeon allows him or her to see surgical problems in a different light and select from a variety of options to solve surgical problems. Plastic surgeons have received broad training, and most have completed residencies in other fields such as general surgery, ENT surgery, orthopedics, urology, or neurosurgery.
The basic principles of plastic surgery are careful analysis of the surgical problem, careful planning of procedures, precise technique, and atraumatic handling of tissues. Alteration, coverage, and transfer of skin and associated tissues are the most common procedures performed. Plastic surgery may deal with closure of surgical wounds—particularly recalcitrant wounds such as those occurring post radiation or poorly healing wounds in immunocompromised patients—removal of skin tumors, repair of soft tissue injuries or burns, correction of acquired or congenital deformities of the breast, or repair of cosmetic defects. Operations on the head and neck and the hand may require special surgical training.
In the past 2 decades, increased knowledge of anatomy and the development of many new techniques have brought about important changes in plastic surgery. It is now known that in many areas the blood supply of the skin is derived principally from vessels arising from underlying muscles and larger perforating blood vessels rather than solely from vessels of the subcutaneous tissue, as was formerly thought. One-stage transfer of large areas of skin and muscle tissue can be accomplished if the axial pedicle of the underlying muscle is included in the transfer. With the use of microsurgical techniques, musculocutaneous units or combinations of bone, muscle, and skin can be successfully transferred and vessels and nerves less than 1 mm in size can be repaired. These so-called free-flap transplantations are a major advance in the treatment of defects that were previously untreatable or required lengthy or multistaged procedures. More sophisticated knowledge of the blood supply to the skin has introduced a concept of perforator flaps whereby one perforating vessel is identified that may supply a large segment of overlying skin. Similarly, the concept of neurocutaneous arteries and flaps has given rise to the design of additional flap territories such as the so-called sural flap in the lower leg.
The plastic surgeon, as a member of the craniofacial surgical team, is able to dramatically improve the appearance and function of children with severe congenital deformities. Children of normal intelligence who previously had been social outcasts are now able to lead relatively normal lives. Improved understanding of facial growth and abnormal development and diagnostic techniques such as the CT scan, MRI, and three-dimensional computer-assisted imaging enable the reconstructive surgeon to develop a complex strategy for remodeling the deformed craniofacial skeleton. This may involve remodeling or repositioning part or all of the cranial vault, the orbits, the mid face, and the mandible. These complex and at times formidable reconstructions, which were performed by moving both units and adding bone grafts from the ileum, have more recently been somewhat simplified with the introduction of the miniplate method of fixation as well as of bone substitutes that may serve as a scaffold upon which normal bone can regrow.
A recent notable advance in craniofacial surgery has been the introduction of distraction osteogenesis, which borrows from the Ilizarov principle of distraction where one makes a cortical cut in the bone and then applies a distraction apparatus so that in measured amounts (usually 1 mm per day) the bone is transported to bridge a gap. In craniofacial surgery it is more commonly brought to bear to enlarge or cause overgrowth of areas such as an underdeveloped mandible.
Additional areas of involvement for the plastic surgeon entail allotransplantation, particularly with the increasing number of clinical limb allotransplants, which unfortunately at present still require immunosuppression. It is hoped that immunotolerance will some day become a reality, thus allowing transplantation of nonessential organs.
Tissue engineering of bone, cartilage, and nerve is an area of ongoing research for plastic surgeons. Although encouraging experimental results have been reported in anatomic areas difficult to reconstruct such as the external ear, there are as yet no clinical applications.
Fetal surgery—an area pioneered by a number of plastic surgeons—appears to be in a quiescent stage, particularly because of advances in the treatment of cleft lip and cleft palate in the newborn.
Jones JW et al: Successful hand transplantation. One-year follow-up. Louisville Hand Transplant Team. N Engl J Med 2000;343:468.
SKIN GRAFTS
A graft of skin detaches epidermis and varying amounts of dermis from its blood supply in the donor area and is placed in a new bed of blood supply from the base of the wound, or recipient area. Although the technique is relatively simple to perform and generally reliable, definite considerations about the donor area and adequacy of the recipient area are important. Skin grafting is a quick, effective way to cover a wound if vascularity is adequate, infection is not present, and hemostasis is assured. Color match, contour, durability of the graft, and donor morbidity must be considered.
Types of Skin Grafts
Skin grafts can be either split-thickness or full-thickness grafts (Figure 44-1). Each type has advantages and disadvantages and is indicated or contraindicated for different kinds of wounds (Table 44-1).
A. Split-Thickness Grafts
Thinner split-thickness grafts (0.01-0.015 inch) become vascularized more rapidly and survive transplantation more reliably. This is important in grafting on less than ideal recipient sites, such as contaminated wounds, burn surfaces, and poorly vascularized surfaces (eg, irradiated sites). A second advantage is that donor sites heal more rapidly and can be reused within a relatively short time (7-10 days) in critical cases such as major burns.
In general, however, the disadvantages of thin split-thickness grafts outweigh the advantages. Thin grafts exhibit the highest degree of postgraft contraction, offer the least amount of resistance to surface trauma, and are least like normal skin in texture, suppleness, pore pattern, hair growth, and other characteristics. Hence, they are usually aesthetically unacceptable.
Thicker split-thickness skin grafts (> 0.015 inch) contract less, are more resistant to surface trauma, and are more similar to normal skin than are thin split-thickness grafts. They are also aesthetically more acceptable but not as acceptable as full-thickness grafts.
The disadvantages of thick split-thickness grafts are relatively few but can be significant. They are less easily vascularized than thin grafts and thus result in fewer successful takes when used on less than ideal surfaces. Their donor sites are slower to heal (requiring 10-18 days) and heal with more scarring than donor sites for thin split-thickness grafts—a factor that may prevent reuse of the area.
Meshed grafts are usually thin or intermediate split-thickness grafts that have been rolled under a special cutting machine to create a mesh pattern. Although grafts with these perforations can be expanded from one and one-half to nine times their original size, expansion to one and one-half times the unmeshed size is the most useful. Meshed grafts are advantageous because they can be placed on an irregular, possibly contaminated wound bed and will usually take. Also, complications of hemostasis are fewer, because blood and serum exude through the mesh pattern. The disadvantage is poor appearance following healing (alligator hide).
Donor sites for split-thickness grafts heal spontaneously by epithelialization. During this process, epithelial cells from the sweat glands, sebaceous glands, or hair follicles proliferate upward and spread across the wound surface. If these three structures are not present, epithelialization will not occur.
B. Full-Thickness Grafts
Full-thickness skin grafts include the epidermis and all the dermis. They are the most aesthetically desirable of the free grafts since they include the highest number of skin appendage elements, undergo the least amount of contracture, and have a greater ability to withstand trauma. There are several limiting factors in the use of full-thickness grafts. Since no epidermal elements remain to produce epithelialization in the donor site, it must be closed primarily, and a scar will result. The size and number of available donor sites is therefore limited. Furthermore, conditions at the recipient site must be optimal in order for transplantation to be successful.
Areas of thin skin are the best donor sites for full-thickness grafts (eg, the eyelids and the skin of the postauricular, supraclavicular, antecubital, inguinal, and genital areas). Submammary and subgluteal skin is thicker but allows camouflage of donor area scars. In grafts thicker than approximately 0.015 inch, the results of transplantation are often poor except on the face, where vascularity is so good.
C. Composite Grafts
A composite graft is also a free graft that must reestablish its blood supply in the recipient area. It consists of a unit with several tissue planes that may include skin, subcutaneous tissue, cartilage, or other tissue. Dermal fat grafts, hair transplant grafts, and skin and cartilage grafts from the ear fall into this category. Obviously, composite grafts must be small or at least relatively thin and will require recipient sites with excellent vascularity. These grafts are generally used in the face.
D. Cultured Epithelial and Dermal Grafts
Epithelial cells were first to be cultured in vitro and made to coalesce into sheets that could be used to cover full-thickness wounds. Although these culture epithelial sheets were used in the treatment of burns, the result was unsatisfactory because the coverage was very fragile and unsatisfactory. More recently, success has been obtained with artificial dermis which when placed in an appropriate bed will revascularize and can then be covered by a very thin (0.05 cm) split-thickness skin graft. This artificial dermis is increasingly being used in the treatment of burns. Modifications of this concept have also been applied to the care of chronic ulcers, particularly in the leg. The artificial dermis is made out of a collagen matrix and has very low or no antigenicity.
Obtaining Skin Grafts
Instruments used for obtaining skin grafts include razor blades, skin grafting knives (Blair, Ferris Smith, Humby, Goulian), manual drum dermatomes (Padgett, Reese), and electric or air-powered dermatomes (Brown, Padgett, Hall). The electric and air-powered dermatomes are the most widely used because of their reliability and ease of operation. A surgeon, even with only limited experience, can successfully obtain sheets of split-thickness skin grafts, using the electric dermatomes.
The Skin Graft Recipient Area
To ensure survival of the graft, there must be (1) adequate vascularity of the recipient bed, (2) complete contact between the graft and the bed, (3) adequate immobilization of the graft-bed unit, and (4) relatively few bacteria in the recipient area.
Since survival of the graft is dependent upon growth of capillary buds into the raw undersurface of the graft, vascularity of the recipient area is of prime importance. Avascular surfaces that will not generally accept free grafts are tissues with severe radiation damage, chronically scarred ulcer beds, bone or cartilage denuded of periosteum or perichondrium, and tendon or nerve without their paratenon or perineurium, respectively. For these surfaces, a bed capable of producing capillary buds must be provided; in some cases, excision of the deficient bed down to healthy tissue is possible. All unhealthy granulation tissue must be removed, since bacterial counts in granulation tissue are often very high. If bone is exposed, it can be decorticated down to healthy cancellous bone with the use of a chisel or power-driven burr, and a meshed split-thickness skin graft can be applied. If an adequate vascular bed cannot be provided or if the presence of essential structures such as tendons or nerves precludes further debridement, skin or muscle flaps are generally indicated for coverage.
Inadequate contact between the graft and the recipient bed can be caused by collection of blood, serum, or lymph fluid in the bed; formation of pus between the graft and the bed; or movement of the graft on the bed.
After the graft has been applied directly to the prepared recipient surface, it may or may not be sutured in place and may or may not be dressed. Whenever the maximum aesthetic result is desired, the graft should be cut exactly to fit the recipient area and precisely sutured into position without any overlapping of edges. Very large or thick split-thickness grafts and full-thickness grafts will usually not survive without a pressure dressing. In areas such as the forehead, scalp and extremities, adequate immobilization and pressure can be provided by circular dressings. Tie-over pressure stent dressings are advisable for areas of the face, where constant pressure cannot be provided by simple wraparound dressings, or areas where movement cannot be avoided, such as the anterior neck, where swallowing causes constant motion; and areas of irregular contour, such as the axilla. The ends of the fixation sutures are left long and tied over a bolus of gauze fluffs, cotton, a sponge, or other suitable material (Figure 44-2).
Grafts applied to freshly prepared or relatively clean surfaces are generally sutured or stapled into place and dressed with pressure. A single layer of damp or other nonadherent fine-mesh gauze is applied directly over the graft. Immediately over this are placed several thicknesses of flat gauze cut in the exact pattern of the graft. On top of these is placed a bulky dry dressing of gauze fluffs, cotton, a sponge, or other material. Pressure is then applied by wraparound dressings, adhesive tape, or a tie-over pressure stent dressing.
In many cases, it is permissible—and sometimes even preferable—to leave a skin graft site open with no dressing. This is particularly true in slightly infected wounds, where the grafts tend to float off in the purulent discharge produced by the wound. These wounds are best treated with meshed grafts, so that liquid forming between the graft and the wound bed can exude and be removed without disturbing the graft. This treatment can also be used for noninfected wounds that produce an unusual amount of serous or lymphatic drainage, as occurs following radical groin dissections.
In severely ill patients, such as those with major burns, where time under anesthesia must be kept to a minimum, large sheets of meshed split-thickness skin grafts are rapidly applied but not sutured. Skin staples may be used to fix the graft rapidly. Grafts need not be dressed if the area is small, but if the area is large or circumferential, a dressing should be applied. Meshed grafts should generally be covered for 24-48 hours to prevent dryness, since their dermal barrier has been partly disrupted.
Various biologic adhesives, in particular autologous fibrin glue, is being used to immobilize skin grafts. This is especially useful in the face or hands, or areas where bandaging is difficult or cumbersome.
Skin graft dressings may be left undisturbed for 5-7 days after grafting if the grafted wound was free of infection, if complete hemostasis was obtained, if fluid collection is not expected, and if immobilization is adequate. If any one of these conditions is not met, the dressing should be changed within 24-48 hours and the graft inspected. If blood, serum, or purulent fluid collection is present, the collection should be evacuated—usually by making a small incision through the graft with a scalpel blade and applying pressure with cotton-tipped applicators. The pressure dressing is then reapplied and changed daily so that the graft can be examined and fluid expressed as it collects.
The Skin Graft Donor Area
The ideal donor site would provide a graft identical to the skin surrounding the area to be grafted. Since skin varies greatly from one area to another as far as color, thickness, hair-bearing qualities, and texture are concerned, the ideal donor site (such as upper eyelid skin to replace skin loss from the opposite upper eyelid) is usually not found. However, there are definite principles that should be followed in choosing the donor area.
A. Color Match
In general, the best possible color match is obtained when the donor area is located close to the recipient area. Color and texture match in facial grafts will be much better if the grafts are obtained from above the region of the clavicles. However, the amount of skin obtainable from the supraclavicular areas is limited. If larger grafts for the face are required, the immediate subclavicular regions of the thorax will provide a better color match than areas on the lower trunk or the buttocks and thighs. When these more distant regions are used, the grafts will usually be lighter in color than the facial skin in whites. In people with dark skin, hyperpigmentation occurs, producing a graft that is much darker than the surrounding facial skin.
B. Thickness of the Graft and Donor Site Healing
Donor sites of split-thickness grafts heal by epithelialization from the epithelial elements remaining in the donor bed. The ability of the donor area to heal and the speed with which it does depends upon the number of these elements present. Donor areas for very thin grafts will heal in 7-10 days, whereas donor areas for intermediate-thickness grafts may require 10-18 days and those for thick grafts 18-21 days or longer.
Since there is a normal anatomic variation in the thickness of skin, donor sites for thicker grafts must be chosen with the potential for healing in mind and should be limited to regions on the body where the skin is thick. Infants, debilitated adults, and elderly people have thinner skin than healthy younger adults. Grafts that would be split-thickness in the normal adult may be full-thickness in these patients, resulting in a donor site that has been deprived of the epithelial elements necessary for healing.
C. Management of the Donor Site
The donor site itself can be considered a clean open wound that will heal spontaneously. After initial hemostasis, the wound will continue to ooze serum for 1-4 days, depending on the thickness of the skin taken. The serum should be collected and the wound kept clean so that healing can proceed at a maximal rate. The wound should be cared for as described above for clean open wounds in either of two ways.
The more common method is the open (dry) technique. The donor site is dressed with porous sterile fine-mesh or nonadherent gauze. After 24 hours, the dry gauze is changed but the nonadherent gauze is left on the wound and exposed to the air, a heat lamp, or a blow dryer. A scab will form on the gauze and will peel off from the edges as epithelialization is completed underneath. This method has the advantage of simple maintenance once the wound is dry.
The second method is the closed (moist) technique. Studies have demonstrated that the rate of epithelialization is enhanced in a moist environment. In contrast to the dry technique, pain can be reduced or virtually eliminated. Moist-to-moist gauze dressings that require frequent wetting have been replaced by newer synthetic materials. A gas-permeable membrane (OpSite) that sticks to the surrounding skin provides an artificial blister over the wound. Occasionally there is a break in the protective seal covering leakage of serum collected under the membrane. This increases the risk of infection, especially in a contaminated zone. Newer hygroscopic dressings actually absorb and retain many times their weight in water. They are permeable to oxygen yet impervious to bacteria. Infection is still a concern, however, because of occasional exposure of the wound during healing.
(Plastic & Reconstructive Surgery - Luis O. Vasconez, MD, & Henry C. Vasconez, MD )
Kishi K, Nakajima H, Tajima S: Differential responses of collagen and glycosaminoglycan syntheses and cell proliferation to exogenous transforming growth factor beta 1 in the developing mouse skin fibroblasts in culture. Br J Plast Surg 1999;52:579.
van Zuijlen PP et al: Graft survival and effectiveness of dermal substitution in burns and reconstructive surgery in a one-stage grafting model. Plast Reconstr Surg 2000;106:615.
Wang JC, To EW: Application of dermal substitute (Integra) to donor site defect of forehead flap. Br J Plast Surg 2000;53:70.
Current Surgical Diagnosis and Treatment, 11th Ed 2003: Lawrence W. Way, Gerard M. Doherty By McGraw-Hill/Appleton & Lange
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